Analyzing quantitative PCR data (& RealTime PCR in general) - practical example & explanation
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YouTubethe bumbling biochemist
Analyzing quantitative PCR data (& RealTime PCR in general) - practical example & explanation
I’ve talked a lot about the theoretical basis for these techniques - using PCR to make lots of copies on a sequence, using fluorescence to measure the copies as they’re made (i.e in Real Time), and seeing how fast the fluorescence rises - but today I want to show you the practical aspects of using that rise in fluorescence to determine how ...
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