However, there's a symbolic reason behind Tobirama's odd fondness for Water Style, one that pertains to his own brother, Hashirama. As Hashirama possesses the Wood Release, Tobirama's Water Style ...

The second time it was used was when Hiruzen Sarutobi, the Third Hokage, was fighting against the reanimated clones of Hashirama and Tobirama Senju. It's unclear how the First Hokage died.