For decades, optimizing annealing temperatures in PCR has pushed the limits of PCR forward to more difficult tasks. At the same time, mastermixes and kits became more powerful and less prone for ...

The Mastercycler nexus GX2 gradient function allows annealing temperature optimization and denaturation temperature optimization. It was evident from the examination of the PCR products arising out of ...

Producing high yields of good-quality PCR products requires a complex combination of optimal chemistry, primer design, template quality, and cycling parameters. The details of these reaction ...

Multiplex PCR is a difficult technique that allows amplification of two or more products in parallel in a single reaction tube. This technology was first described by Chamberlain et al., in 1988, and ...

In May 1983, Kary Mullis, a young technician working for the Cetus Corporation, sat with idle hands and a busy mind. The laboratory he worked in was attempting to study DNA mutations linked to sickle ...