A nested polymerase chain reaction (PCR) protocol was developed for detecting the presence of Schistosoma mansoni sporocysts in intermediate host snails of the genus Biomphalaria. To accomplish this, ...

The use of new powerful nested polymerase chain reaction (PCR) techniques to identify and screen for prevalence of parasites has a huge potential. It allows for the detection and identification of low ...

Therefore, the reported enhanced sensitivity of the competitive quantitative nested RT-PCR assay by Guo et al may essentially be caused by secondary disturbances of the fluorogenic Taqman assay ...