Protein contributes to the absorbance at 260 nm, artificially increasing the nucleic acid concentration, and is detected by noting a decrease in the 260/280 purity ratio. Figure 1.

For example, enter "Concentration" and "Absorbance." Fill in both columns of the table with the corresponding values for your measurements.

Where c= concentration, A= absorption, ε = molar absorption coefficient, l=optical pathway (path length of the cuvette). For a cuvette having 1cm path length, the formulae to be used are: ...

A method of r e a l time oil concentration measurment has been developed utilizing the effect of ultraviolet light absorption by lubricating oil in the liquid refrigerant line of an automotive air ...